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The PPI-hotspotID Prediction Server

Jon D. Wright1, Chen Yao-Chi1, Chen Yu-Hsien2, Karen Sargsyan1 and Carmay Lim1,2

1Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
2Department of Chemistry, National TsingHua University, HsinChu, Taiwan

This server can be used to predict PPI-hotspotID residues and for a given protein chain, the method it uses is summarized in this pdf. For your target protein provide either a single chain PDB file or give the 4 letter PDB code and the chain ID that you would like a prediction for. (Note: Currently we are taking only single chain PDB structures).

If you wish, you can view a sample output that was given from a saved prediction we did earlier for 1A5E chain A. Output from a previous prediction on chain A from 1A5E can be found here.

Submission area choose option A or Option B:

Option A) For a single protein chain structure that is to be uploaded from a file.
Select pdb file to upload:
then click on the 'Upload File'

Option B) For known PDB archive entry with a protein chain.
Provide a PDB ID (4 chars):
and a chain (1 char) to upload:
then click on the submit button

PPI-hotspotID was created by Jon D. Wright, Chen Yao-Chi, Chen Yu-Hsien, Karen Sargsyan and Carmay Lim and is hosted in the Institute of BioMedical Sciences, Academia Sinica, Taipei, Taiwan.

Code that this server uses can be obtained from github as wrigjz/ppihotspotid and wrigjz/conservation_python.

The original manuscript publication in eLife is available from here, from this website you can download supplementary figures and tables S1 and S2.

PPI-hotspotID is hosted at The Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan.